NOTE:
Mix the alcohol and acetic acid together. Slowly add the hematoxylin crystals
while stirring until they are all dissolved.
2 Solution B.
Absolute ethanol .......................................... 90 ml
Glycerin ....................................................... 100 ml
NOTE:
Mix the alcohol and glycerin in a separate container from the one above.
STEP 1: Add solution A to solution B.
STEP 2: Dissolve 10 grams of aluminum potassium sulfate in 100 milliliters of distilled
water with heat.
STEP 3: Cool the solution and add the alum solution to the hematoxylin-alcohol-acid-
glycerin mixture. Stir constantly.
STEP 4: Expose the solution to light and air. Allow the solution to ripen for four to six
weeks.
STEP 5: Ensure that the ripe solution has the color of port wine. The ripening process
is hastened by standing the solution in a warm place and shaking it
occasionally.
(b) Alcohol-ether mixture.
Ethyl alcohol, 95 percent ................................... 50 ml
Ether, anhydrous ............................................... 50 ml
(c)
Formalin solution, two percent.
Formaldehyde ................................................... 2 ml
Distilled water .................................................... 100 ml
(2)
Procedure.
STEP 1: Draw one milliliter of blood by venipuncture and mix it with 10 milliliters of a
two percent solution of formalin. Mix, store, and/or ship the sample to the
laboratory. The formalin will lake the red blood cells.
STEP 2: Centrifuge the sample for five minutes at 1,000 revolutions per minute (rpm).
STEP 3: Pour off the supernatant without disturbing the sediment. With a capillary
pipet, transfer a portion of the sediment to a clean slide.
MD0841
2-22