Quantcast Brine flotation. - Parasitology I

Share on Google+Share on FacebookShare on LinkedInShare on TwitterShare on DiggShare on Stumble Upon
Custom Search
 
  
 
(c)
Wire loop method of removing the concentrate (preferred method).
STEP 1: Prepare a bacteriological loop so it is at a 90-degree angle to the wire.
STEP 2: Gently slide the loop just under the surface of the liquid and remove three to
four loopsfull of the concentrate.
STEP 3: Mix with a drop of the desired stain and examine for parasites.
(d) Coverslip method of removing the concentrate (alternate method).
STEP 1: Carefully raise the fluid level of the tube until a positive miniscus is obtained
(adding the solution down the side of the test tube prevents remixing of the
surface concentrate.
STEP 2: Place a coverslip on top of the tube touching the meniscus and allow to stand
undisturbed for 10 to 15 minutes.
STEP 3: With a quick motion, remove the coverslip and place it on a prepared slide to
which one drop of the desired stain has been added.
STEP 4: Examine for parasites.
(2)  Brine flotation. This procedure is easy to perform and it is used for the
recovery of ova other than operculate and Schistosoma. Trophozoites are destroyed and
cysts are distorted beyond recognition. The method is recommended for the screening of
hookworms in a less than suitable environment.
(a) Reagent. Make a saturated solution of sodium chloride (table salt
suffices) in boiling tap water. Allow to cool and check the specific gravity to make sure
that it is at least 1:20. If the specific gravity is too low, reboil the solution and add more
salt. Filter and store in a cool place.
(b) Procedure.
STEP 1: Make a 1:1 mixture of feces and the brine solution in a disposable container.
STEP 2: Transfer to a 20 milliliter round test tube (about one inch in diameter) and fill
the tube to the brim with salt solution.
STEP 3: Collect the concentrate in the same manner as the zinc sulfate method.
STEP 4: Observe microscopically for parasites.
MD0841
2-46



Medical News
Breast milk reveals clues for health
Evidence shows that breast-feeding is good for babies, boosting immunity...
medicalxpress.com
Research offers new hope for treatment of rare genetic disease
New research by experts at The University of Nottingham could...
medicalxpress.com
Two ears are better than one
Hearing-impaired children fitted with a second cochlear implant (CI) early...
medicalxpress.com
Neuroscientist develops brain vitality index
Why is it we are happy to talk about our...
medicalxpress.com
Promising new antibiotic defies conventional wisdom on treating superbug
A new antibiotic being developed at the University of Virginia...
medicalxpress.com
Cooler bedroom temperatures may boost metabolic activity
A new study has found that turning the thermostat down...
medicalxpress.com
Children are silent victims of the 'hidden epidemic' of tuberculosis
Nations of the world came together in 2000 to make...
medicalxpress.com
Internet search engines as both an aid and a danger for people at risk of suicide
If someone is thinking about suicide, the Internet often serves...
medicalxpress.com
Researchers find new fertility drug to be more effective than one used for past 40 years
A fertility drug used for more than four decades could...
medicalxpress.com
Yellow fever epidemic presents 200-year-old lesson in crisis management
Among natural and man-made disasters, there is perhaps nothing more...
medicalxpress.com
Global impact of the Ebola outbreak
The Ebola virus has been spreading in West Africa since...
medicalxpress.com
Simple test helps doctors catch more concussions on the field
On the football field, sometimes the signs of a concussion...
medicalxpress.com
Researchers find one third of cancer patients are killed by a 'fat-burning' process related to obesity
Most cancer researchers are working on the biology of the...
medicalxpress.com
S.Korea detects second foot-and-mouth case
South Korea on Monday reported its second case of foot-and-mouth...
medicalxpress.com
Oncology fellows, clinicians report similar burnout
(HealthDay)—U.S. oncology fellows may underestimate the workload they will experience...
medicalxpress.com
Ebola kills Liberian doctor, 2 Americans infected
(AP)—One of Liberia's most high-profile doctors has died of Ebola,...
medicalxpress.com
Virus drugmaker fights pediatricians' new advice
(AP)—A drugmaker is clashing with pediatricians over new recommendations to...
medicalxpress.com
Potassium pills, implantable device help heart-failure patients cope with summer heat
Patients with heart failure engage in a delicate balancing act...
medicalxpress.com
Hepatitis C virus genotype 1 is most prevalent worldwide
In one of the largest prevalence studies to date, researchers...
medicalxpress.com
1 in 3000 blood donors in England infected with hepatitis E
The first systematic analysis of hepatitis E virus (HEV) transmission...
medicalxpress.com
 


Privacy Statement - Copyright Information. - Contact Us

comments powered by Disqus

Integrated Publishing, Inc.
9438 US Hwy 19N #311 Port Richey, FL 34668

Phone For Parts Inquiries: (727) 755-3260
Google +