Quantcast Brine flotation. - Parasitology I

Share on Google+Share on FacebookShare on LinkedInShare on TwitterShare on DiggShare on Stumble Upon
Custom Search
 
  
 
(c)
Wire loop method of removing the concentrate (preferred method).
STEP 1: Prepare a bacteriological loop so it is at a 90-degree angle to the wire.
STEP 2: Gently slide the loop just under the surface of the liquid and remove three to
four loopsfull of the concentrate.
STEP 3: Mix with a drop of the desired stain and examine for parasites.
(d) Coverslip method of removing the concentrate (alternate method).
STEP 1: Carefully raise the fluid level of the tube until a positive miniscus is obtained
(adding the solution down the side of the test tube prevents remixing of the
surface concentrate.
STEP 2: Place a coverslip on top of the tube touching the meniscus and allow to stand
undisturbed for 10 to 15 minutes.
STEP 3: With a quick motion, remove the coverslip and place it on a prepared slide to
which one drop of the desired stain has been added.
STEP 4: Examine for parasites.
(2)  Brine flotation. This procedure is easy to perform and it is used for the
recovery of ova other than operculate and Schistosoma. Trophozoites are destroyed and
cysts are distorted beyond recognition. The method is recommended for the screening of
hookworms in a less than suitable environment.
(a) Reagent. Make a saturated solution of sodium chloride (table salt
suffices) in boiling tap water. Allow to cool and check the specific gravity to make sure
that it is at least 1:20. If the specific gravity is too low, reboil the solution and add more
salt. Filter and store in a cool place.
(b) Procedure.
STEP 1: Make a 1:1 mixture of feces and the brine solution in a disposable container.
STEP 2: Transfer to a 20 milliliter round test tube (about one inch in diameter) and fill
the tube to the brim with salt solution.
STEP 3: Collect the concentrate in the same manner as the zinc sulfate method.
STEP 4: Observe microscopically for parasites.
MD0841
2-46



Medical News
Researcher disputes claim that humans can distinguish one trillion odors
An Arizona State University researcher is calling into question recent...
medicalxpress.com
Study shows second severe allergic reaction can occur hours after first
Parents of kids with severe allergies know how scary a...
medicalxpress.com
Researchers find key mechanism that causes neuropathic pain
Scientists at the University of California, Davis, have identified a...
medicalxpress.com
Deep sea light shines on drug delivery potential
A naturally occurring bioluminescent protein found in deep sea shrimp—which...
medicalxpress.com
Can four fish oil pills a day keep the doctor away?
Fish oil is one of the most popular dietary supplements...
medicalxpress.com
Policies on children's radio frequency exposure confusing
New research suggests guidelines on children's exposure to radio frequency...
medicalxpress.com
Suppressing the immune response may lead to more potent vaccines, a study finds
Yale researchers uncovered a new role for a type of...
medicalxpress.com
New media helps emergency physicians continue care beyond the ER
Yale researchers find a critical mass of patients who see...
medicalxpress.com
Study of tobacco company RRPs reveals tax increases applied unequally
New Zealand's largest tobacco company is not keeping the recommended...
medicalxpress.com
Discovery of new genomic aberrations of gastric cancer could pave the way for precision medicine
Singapore scientists from A*STAR's Genome Institute of Singapore (GIS) and...
medicalxpress.com
Why can't we stop cholera in Haiti?
In early February, when Jenniflore Abelard arrived at her parents'...
medicalxpress.com
Five tips for bringing your elderly loved one to the emergency department
Getting old is tough. Taking care of an aging loved...
medicalxpress.com
Child bullies most often pick on others for 'being fat'
A new multi-national study by the Rudd Center for Food...
medicalxpress.com
Marine sponge shows tumour-stunting promise
The research, which has been published in the highly-regarded journal...
medicalxpress.com
Psychiatrist discusses guidelines for treating teens' eating disorders
Eating disorders have been described in medical literature since the...
medicalxpress.com
Transgenic mosquito works to control dengue-carrying mosquitos
Releases of the genetically engineered Oxitec mosquito, commonly known as...
medicalxpress.com
Potential treatment option for children with Ewing's sarcoma
A research consortium made up of the Virgen del Rocio...
medicalxpress.com
The battle for control of the human breast milk industry
It could trade for 400 times more than t7/8he price...
medicalxpress.com
The next anti-tuberculosis drug may already be in your local pharmacy
Testing thousands of approved drugs, EPFL scientists have identified an...
medicalxpress.com
Study identifies new way to kill the malaria parasite
Scientists have discovered new ways in which the malaria parasite...
medicalxpress.com
 


Privacy Statement - Copyright Information. - Contact Us

comments powered by Disqus

Integrated Publishing, Inc.
9438 US Hwy 19N #311 Port Richey, FL 34668

Phone For Parts Inquiries: (727) 755-3260
Google +