2 Mordant--solution B.
Ferric Ammonium Sulfate (violet crystals) ... 2.0 gm
Distilled water .............................................. 50.0 ml
Break the crystals into small pieces and mix with the water until
dissolved.
3 Hematoxylin stain.
a Stock solution. Dissolve 10.0 grams of hematoxylin powder in
100 milliliters of 95 percent ethanol. Store in the presence of light for six weeks or longer
(aging). Filter prior to use.
b Working solution--solution C. Dilute five milliliters of the stock
solution with 95 milliliters of distilled water. Prepare this solution daily.
4. Phosphotungstic acid--solution D.
Phosphotungstic acid.........................2.0 gm
Distilled water............................100.0 ml
5. Ethanol 70% with lithium carbonate.
a Stock solution. Dissolve sufficient lithium carbonate in 100
milliliters of distilled water to make a saturated solution. Mix well and save in a brown
stoppered glass bottle.
b Working solution--solution E. Add six to 10 drops of stock
solution for every 100 milliliters of 70 percent ethanol used. Prepare this solution just
prior to use.
(b) Procedure.
STEP 1: Prepare at least five smears from each fresh specimen by streaking the feces
on a clean slide with an applicator stick. Make the smear thin, but do not leave
clear areas on the slide. The optimum smear should be about one inch long.
STEP 2: Fix the wet smears in Schaudinn's fixative for one to 24 hours. Do not allow
the smear to dry. The fixation time may be shortened to five minutes if the
fixative is heated to 50.
STEP 3: Set up a series of jars suitable for staining with the required reagents.
STEP 4: Transfer the slides singly or in racks from dish to dish.
MD0841
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