3 Weakly active cells may take the full 2 minutes to agglutinate.
Do not expect reactions to be as rapid as with ABO reagents.
4 Failure to identify reagents at time of use may allow albumin,
anti-globulin serum, or some other colorless reagent to be added instead of anti-Rho(D).
Capillary testing. See special methods section.
(a) Most slide and rapid tube anti-Rho(D) serums can be used with
saline-suspended cells or with cells in serum, but this should be confirmed by reading
the manufacturer's instructions before use. Table 2-13 illustrates the kinds of
differences that exist in manufacturer's instructions for use of anti-Rho(D).
1 STEP 1: Place one drop of antiserum in labeled tube for test.
2 STEP 2: Place one drop of albumin or control medium in labeled
tube for control.
Table 2-13. Variables in manufacturer's instructions for Rho(D)-testing.