(2)
Example 2: Mixture of antibodies. Table 1-5.
Patient's Group: A1 RhO (Dccee)
Antigenic Determinants
Results
RhO
rh' rh" hr' hr"
Saline
En-
a
b
Panel cell
D
CEce
M N K k Le Le RT 37C HP AGT zyme
1.
+
0
0
+
+
+
+
0
+
0
0
0
0
0
0
0
2.
0
0
0
+
+
+
+
0
+
+
0
+
SH
2+
2+
H
3.
0
+
0
+
+
0
+
0
+
0
+
0
0
0
0
0
4.
+
+
0
0
+
+
+
+
0
0
0
0
0
0
0
0
5.
+
0
+
+
0
+
0
0
+
0
+
0
0
3+
4+
4+
6.
0
0
+
+
+
0
+
0
+
+
0
+
SH
3+
4+
H
Autologous +
0
0
+
+
+
+
0
+0
0
0
0
0
0
0
cells
RT = room temperature; HP = high protein; AGT = = antiglobulin test; 0 = no agglutination;
SH = slight hemolysis; H = hemolysis
Table 1-5. Example of multiple antibodies identified with a simplified cell panel.
Preliminary detection tests:
Saline
4C
RT
37C
0
+
Slight Hemolysis
High Protein
2+
AGT
3+
Enzyme-partial hemolysis. The cells not hemolyzed are
agglutinated.
(a) STEP 1. Select the first RBC sample that did not react with the
serum, in this example, number one. Cross Out all the antigenic determinants present
in this sample.
D C E c e M N K K Lea Leb
(b) STEP 2. Repeat with the next negative sample (number three),
thus eliminating (C) and Le.
C E K Lea Leb
MD0846
1-43
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