Quantcast Cold Antibodies - Immunohematology and Blood Banking II

Share on Google+Share on FacebookShare on LinkedInShare on TwitterShare on DiggShare on Stumble Upon
Custom Search
 
  
 
(2)  Variation in the strength of the antigen. Some antigens that do not show
dosage in terms of zygosity may vary in strength on cells from different individuals. The
antigens most notable for this are P1, Sda, Lea, Leb, and I. Sometimes diminished
antigenic strength can be related to disease, or to the length of time that the cell
suspension has been stored.
1-38. COLD ANTIBODIES
a. Background. These antibodies react best at room temperature (RT) and
below, while reactions after 37C incubation and with AGT are negative or weakly
positive. Some cold agglutinating antibodies, notably anti-I, anti-lH, and anti-H, bind
complement following the antigen-antibody interaction at low temperatures; subsequent
37C incubation may cause the antibody to elute from the cell, but the attached
complement remains, and produces a positive reaction when antiglobulin serum is
added. An antibody displaying these characteristics in the screening test or in
crossmatches should be tested at 18C and/or 4C with specially selected cells, such as
cord cells.
b. Cold Autoabsorption to Remove Cold-Reactive Autoantibody.
(1)  When a strong, cold, autoantibody is present, meaningful
antibody-screening and compatibility studies cannot be done until the autoantibody is
removed. This can be done with the patient's untreated or enzyme-treated cells.
Enzyme pretreatment results in faster antibody removal, and is more effective with very
high-titered autoantibodies. Untreated autoabsorption is usually satisfactory for
removing low-level activity.
(2)  Some alloantibody may also be removed by the Matuhasi-Ogata
phenomenon, or by transfused cells in the circulation. In recently transfused patients,
when autoabsorption might result in removing alloantibody activity, the
antibody-screening test and compatibility tests should be done at 37C, using serum
from a blood sample clotted at 37C and kept at that temperature until the serum is
removed. This permits detection of antibodies active at 37C, and by AGT, but not
cold-reactive alloantibodies.
(a) STEP 1. Obtain a blood specimen from the patient. At the bedside
draw two specimens of blood, one in a tube immediately placed in ice, the other in a
tube containing anticoagulant and incubate it at once at 37C. To do this, bring a
heated block, water bath, or vacuum jug of 37C water to the patient's bedside. The
tube may also be transported in an inside pocket.
(b) STEP 2. Allow the first (iced) specimen to clot in the refrigerator.
Separate the serum by centrifuging in ice-filled cups or a refrigerated centrifuge.
Immediately remove the serum from the clot.
MD0846
1-52



Medical News
Immigrant children given adult dose of hepatitis A vaccine
About 250 immigrant children were given an adult dose of...
medicalxpress.com
Second-line cetuximab active beyond progression in quadruple wild-type patients with mCRC
Patients with metastatic colorectal cancer (mCRC) that are mutation-free in...
medicalxpress.com
MERS cases keep coming from Samsung hospital
Another doctor at a major hospital that has been the...
medicalxpress.com
Doc Shortages, Better Bedside Manner: Healthcare Career Insights
(MedPage Today) -- Must reads about the latest news and...
medpagetoday.com
Study Predicts Huge Toll of Hep C Drugs on Calif. Budget
(MedPage Today) -- Cost estimates range from $512 million to...
medpagetoday.com
Genes Not Responsible for Migraine-Heart Disease Link (CME/CE)
(MedPage Today) -- Migraine without aura actually shares cardioprotective gene...
medpagetoday.com
REM sleep critical for young brain development; medication interferes
Rapid eye movement or REM sleep actively converts waking experiences...
medicalxpress.com
Body Size Not a Factor in Ovarian Ca Dosing (CME/CE)
(MedPage Today) -- Relative dose intensity had stronger link to...
medpagetoday.com
Tweet of the Week: Twitter Banter Escalates Into Pun Battle
(MedPage Today) -- A scientific porcupine named Bill? Nye-quill. Well,...
medpagetoday.com
Liberia works to contain Ebola, find source of new cases
Liberia is working hard to contain Ebola and find the...
medicalxpress.com
Happy Fourth of July from Stanford Medicine
Happy Fourth of July! Our office is closed in honor...
scopeblog.stanford.edu
CMS Floats Outpatient Payment Update, FDA Eyes Codeine Cough Syrups
(MedPage Today) -- Also: House members question CMS fraud prevention...
medpagetoday.com
Noise from fireworks threatens young ears
(HealthDay)—The Fourth of July weekend is a time for celebrations...
medicalxpress.com
Many new teen drivers 'crash' in simulated driving task
(HealthDay)—Around four in 10 newly licensed teen drivers "crashed" in...
medicalxpress.com
Telehealth and High Deductibles Slow Health Spending
(MedPage Today) -- Health spending in employer-based market is trending...
medpagetoday.com
Brain folding related to surface area and thickness, not number of neurons
(Medical Xpress)—A pair of researchers with Universidade Federal do Rio...
medicalxpress.com
New test could predict arthritis drug failure in patients
A study of 311 patients by The University of Manchester...
medicalxpress.com
Cohen's Brain Bits: Where is the Love?
(MedPage Today) -- How neurology could better serve the lesbian,...
medpagetoday.com
Documenting how dementia sufferers benefit from GPS
A brand new study of 200 dementia sufferers in Norway...
medicalxpress.com
It takes a special kind of cyclist to win the Tour de France
The Tour de France is the world's most famous cycling...
medicalxpress.com
 


Privacy Statement - Copyright Information. - Contact Us

comments powered by Disqus

Integrated Publishing, Inc.
9438 US Hwy 19N #311 Port Richey, FL 34668

Phone For Parts Inquiries: (727) 755-3260
Google +