Quantcast
Cold Antibodies - Immunohematology and Blood Banking II

Share on Google+Share on FacebookShare on LinkedInShare on TwitterShare on DiggShare on Stumble Upon
Custom Search
 
  
 
(2)  Variation in the strength of the antigen. Some antigens that do not show
dosage in terms of zygosity may vary in strength on cells from different individuals. The
antigens most notable for this are P1, Sda, Lea, Leb, and I. Sometimes diminished
antigenic strength can be related to disease, or to the length of time that the cell
suspension has been stored.
1-38. COLD ANTIBODIES
a. Background. These antibodies react best at room temperature (RT) and
below, while reactions after 37C incubation and with AGT are negative or weakly
positive. Some cold agglutinating antibodies, notably anti-I, anti-lH, and anti-H, bind
complement following the antigen-antibody interaction at low temperatures; subsequent
37C incubation may cause the antibody to elute from the cell, but the attached
complement remains, and produces a positive reaction when antiglobulin serum is
added. An antibody displaying these characteristics in the screening test or in
crossmatches should be tested at 18C and/or 4C with specially selected cells, such as
cord cells.
b. Cold Autoabsorption to Remove Cold-Reactive Autoantibody.
(1)  When a strong, cold, autoantibody is present, meaningful
antibody-screening and compatibility studies cannot be done until the autoantibody is
removed. This can be done with the patient's untreated or enzyme-treated cells.
Enzyme pretreatment results in faster antibody removal, and is more effective with very
high-titered autoantibodies. Untreated autoabsorption is usually satisfactory for
removing low-level activity.
(2)  Some alloantibody may also be removed by the Matuhasi-Ogata
phenomenon, or by transfused cells in the circulation. In recently transfused patients,
when autoabsorption might result in removing alloantibody activity, the
antibody-screening test and compatibility tests should be done at 37C, using serum
from a blood sample clotted at 37C and kept at that temperature until the serum is
removed. This permits detection of antibodies active at 37C, and by AGT, but not
cold-reactive alloantibodies.
(a) STEP 1. Obtain a blood specimen from the patient. At the bedside
draw two specimens of blood, one in a tube immediately placed in ice, the other in a
tube containing anticoagulant and incubate it at once at 37C. To do this, bring a
heated block, water bath, or vacuum jug of 37C water to the patient's bedside. The
tube may also be transported in an inside pocket.
(b) STEP 2. Allow the first (iced) specimen to clot in the refrigerator.
Separate the serum by centrifuging in ice-filled cups or a refrigerated centrifuge.
Immediately remove the serum from the clot.
MD0846
1-52



Medical News
Death rates from pancreatic cancer predicted to rise in Europe in 2014
Pancreatic cancer is the only cancer for which deaths are...
medicalxpress.com
Genetics explain why some boys and girls are bigger than others
The influence of genetic factors on differences between children's Body...
medicalxpress.com
Bioethicists use theatrical narratives to bridge the gap between society and science
A pair of bioethics experts have added "playwright" to their...
medicalxpress.com
The best toxicology lab: a mouse with a human liver
A few years ago, Stanford pharmacogenomic expert Gary Peltz, MD,...
scopeblog.stanford.edu
How Great the Breast Ca Risk After Radiation as Child?
(MedPage Today) -- Women who survived childhood cancers treated with...
medpagetoday.com
Income Tax Fraud Appears to Target Docs
(MedPage Today) -- Hundreds of doctors across several states and...
medpagetoday.com
Stem cells in circulating blood affect cardiovascular health, study finds
New research suggests that attempts to isolate an elusive adult...
medicalxpress.com
Rural microbes could boost city dwellers' health
The greater prevalence of asthma, allergies and other chronic inflammatory...
medicalxpress.com
FDA Considers Ban on 'Aversive' Shock Devices
(MedPage Today) -- An FDA advisory committee will consider on...
medpagetoday.com
Big Data in Biomedicine technical showcase to feature companies’ innovations related to big data
In an effort to spark collaboration among thought leaders across...
scopeblog.stanford.edu
People with mild cognitive impairment may die at higher rate than people without condition
Mayo Clinic research studying the relationship between death and the...
medicalxpress.com
Marijuana use may increase heart complications in young, middle-aged adults
Marijuana use may result in cardiovascular-related complications—even death—among young and...
medicalxpress.com
People with more education may recover better from traumatic brain injury
People with more years of education may be better able...
medicalxpress.com
Iron consumption can increase risk for heart disease, study says
A new study from the Indiana University School of Public...
medicalxpress.com
Cyber buddy is better than 'no buddy'
A Michigan State University researcher is looking to give exercise...
medicalxpress.com
Study Suggests Heart Risk From Smoking Pot (CME/CE)
(MedPage Today) -- A French study provided support for an...
medpagetoday.com
Pollutants from coal-burning stoves strongly associated with miscarriages in Mongolia
Burning coal for domestic heating may contribute to early fetal...
medicalxpress.com
ASTRO issues guideline on the role of postoperative radiation therapy for endometrial cancer
The American Society for Radiation Oncology (ASTRO) has issued a...
medicalxpress.com
MERS Watch: Saudi Numbers Up Again
(MedPage Today) -- Saudi Arabia is reporting 11 new cases...
medpagetoday.com
Review: sugar-sweetened drink intake tied to elevated BP
(HealthDay)—Sugar-sweetened beverage (SSB) intake is associated with elevated blood pressure...
medicalxpress.com
   


Privacy Statement - Copyright Information. - Contact Us

comments powered by Disqus

Integrated Publishing, Inc.
9438 US Hwy 19N #311 Port Richey, FL 34668

Phone For Parts Inquiries: (727) 755-3260
Google +