Quantcast One-Tube Crossmatch Procedure. - Immunohematology and Blood Banking II

Share on Google+Share on FacebookShare on LinkedInShare on TwitterShare on DiggShare on Stumble Upon
Custom Search
 
  
 
c. One-Tube Crossmatch Procedure.
(1)
STEP 1. Place two drops of recipient serum in a labeled tube.
STEP 2. Add one drop of a 2 to 5 percent saline suspension of donor
(2)
cells.
(3)  STEP 3. Centrifuge; examine for hemolysis, agglutination; grade and
record results.
NOTE:
Cold agglutinins may cause difficulty with this procedure. The two-tube
crossmatch may resolve the difficulty.
(4)  STEP 4. Add 2 to 3 drops of 22 to 30 percent albumin according to
manufacturer's directions; mix. Centrifuge, incubate 15 to 60 minutes at 37C, read and
record the results.
(5)  STEP 5. Centrifuge immediately upon removing from the incubator;
examine for hemolysis, agglutination; grade and record results.
(6)  STEP 6. Wash three or four times with saline. After last wash, decant
completely. Add 1 to 2 drops of antiglobulin serum and mix.
(7)  STEP 7. Centrifuge, examine for agglutination, grade and record the
results. (Use of optical aid is optional at this step.)
(8)  STEP 8. Add one drop of known sensitized cells to all negative tests.
Centrifuge, examine for agglutination, and record result. If no agglutination is seen, the
antiglobulin phase must be repeated. If no hemolysis or agglutination is seen in any
phase, the blood is considered compatible.
d. Two-Tube Crossmatch Procedure.
STEP 1. Place two drops of recipient serum in each of two labeled
(1)
tubes.
(2)  STEP 2. Add one drop of a 2 to 5 percent saline suspension of donor
cells to each tube, and mix. Centrifuge, read, and record results.
STEP 3.
(3)
(a) Incubate one tube 15 to 30 minutes at room temperature.
(b) Centrifuge; examine for hemolysis, agglutination; read and record
results.
(c)
Discard tube.
MD0846
1-68



Medical News
PodMed: A Medical News Roundup From Johns Hopkins (with audio)
(MedPage Today) -- This week's topics include Ebola face-to-face, the...
medpagetoday.com
Ig Nobel Winners, Bad News Sandwich: That's Improbable!
(MedPage Today) -- A weekly report from our friends at...
medpagetoday.com
Grand Roundup: Top posts for the week of Sept. 14
The five most-read stories this week on Scope were: So...
scopeblog.stanford.edu
For Autistic Adults, Coverage Options Are Scarce
(MedPage Today) -- Once someone with autism turns 21, "they...
medpagetoday.com
Scientists discover an on/off switch for aging cells
(Medical Xpress)—Scientists at the Salk Institute have discovered an on-and-off...
medicalxpress.com
Ultrasound enhancement provides clarity to damaged tendons, ligaments
The potential was hidden in plain sight, pretty much since...
medicalxpress.com
Sierra Leone faces criticism over Ebola shutdown
Sierra Leone began the second day of a 72-hour nationwide...
medicalxpress.com
New cancer drug target involving lipid chemical messengers
More than half of human cancers have abnormally upregulated chemical...
medicalxpress.com
New Regimens Boost Optimism for Pancreatic Ca (CME/CE)
SAN FRANCISCO (MedPage Today) -- Stereotactic ablative radiation allowed 40%...
medpagetoday.com
This Week: Ebola, Interns, and Low-T Tx
(MedPage Today) -- An exclusive piece on the rising costs...
medpagetoday.com
Twenty-four percent of middle-aged and older Americans meet muscle-strengthening guidelines
Past research has shown that strength training can benefit older...
scopeblog.stanford.edu
Twenty-four percent of middle-aged and older Americans meet muscle-strengthening guidelines
Past research has shown that strength training can benefit older...
scopeblog.stanford.edu
Vitamin K antagonist plus clopidogrel feasible for PCI
(HealthDay)—Vitamin K antagonists (VKA) combined with clopidogrel may be a...
medicalxpress.com
Electronic health records tied to shorter time in ER
(HealthDay)—Length of emergency room stay for trauma patients is shorter...
medicalxpress.com
Tight blood sugar control doesn't prevent strokes in diabetics
(HealthDay)—A six-year study of people with type 2 diabetes found...
medicalxpress.com
Friday Feedback: Docs Defend Fee-for-Service
(MedPage Today) -- Are American healthcare providers ready to let...
medpagetoday.com
Presence of peers ups health workers' hand hygiene
(HealthDay)—The presence of other health care workers improves hand hygiene...
medicalxpress.com
Surgical Training Can Be Fun and Games
(MedPage Today) -- Competition and prizes motivated surgical residents to...
medpagetoday.com
UN: Ebola 'Threat to Security'
(MedPage Today) -- The West Africa Ebola epidemic is a...
medpagetoday.com
Study explores docs' roles in end-of-life hospitalizations
(HealthDay)—Family physicians have several distinct roles in preventing and guiding...
medicalxpress.com
 


Privacy Statement - Copyright Information. - Contact Us

comments powered by Disqus

Integrated Publishing, Inc.
9438 US Hwy 19N #311 Port Richey, FL 34668

Phone For Parts Inquiries: (727) 755-3260
Google +