Quantcast One-Tube Crossmatch Procedure. - Immunohematology and Blood Banking II

Share on Google+Share on FacebookShare on LinkedInShare on TwitterShare on DiggShare on Stumble Upon
Custom Search
c. One-Tube Crossmatch Procedure.
STEP 1. Place two drops of recipient serum in a labeled tube.
STEP 2. Add one drop of a 2 to 5 percent saline suspension of donor
(3)  STEP 3. Centrifuge; examine for hemolysis, agglutination; grade and
record results.
Cold agglutinins may cause difficulty with this procedure. The two-tube
crossmatch may resolve the difficulty.
(4)  STEP 4. Add 2 to 3 drops of 22 to 30 percent albumin according to
manufacturer's directions; mix. Centrifuge, incubate 15 to 60 minutes at 37C, read and
record the results.
(5)  STEP 5. Centrifuge immediately upon removing from the incubator;
examine for hemolysis, agglutination; grade and record results.
(6)  STEP 6. Wash three or four times with saline. After last wash, decant
completely. Add 1 to 2 drops of antiglobulin serum and mix.
(7)  STEP 7. Centrifuge, examine for agglutination, grade and record the
results. (Use of optical aid is optional at this step.)
(8)  STEP 8. Add one drop of known sensitized cells to all negative tests.
Centrifuge, examine for agglutination, and record result. If no agglutination is seen, the
antiglobulin phase must be repeated. If no hemolysis or agglutination is seen in any
phase, the blood is considered compatible.
d. Two-Tube Crossmatch Procedure.
STEP 1. Place two drops of recipient serum in each of two labeled
(2)  STEP 2. Add one drop of a 2 to 5 percent saline suspension of donor
cells to each tube, and mix. Centrifuge, read, and record results.
(a) Incubate one tube 15 to 30 minutes at room temperature.
(b) Centrifuge; examine for hemolysis, agglutination; read and record
Discard tube.

Medical News
Autistic features linked to prenatal exposure to fire retardants, phthalates
Exposure during pregnancy to a combination of fire retardant chemicals...
Exposure to endocrine disruptors during pregnancy affects the brain two generations later
Prenatal exposure to low doses of the environmental contaminants polychlorinated...
Are your cells stressed out? One Stanford researcher is helping them relax
Welcome to Biomed Bites, a weekly feature that introduces readers...
Maker of device in 'superbug' outbreak lacked FDA clearance
The manufacturer of a medical instrument at the center of...
Mutation in APC2 gene causes Sotos features
Sotos syndrome is a congenital syndrome that is characterized by...
Abnormal brain rhythms tied to problems with thinking in schizophrenia
By studying specially bred mice with specific developmental and cognitive...
If you come from a family with relatives who have lived long lives, you will too?
Recent research from the Long Life Family Study (LLFS) confirms...
Drug to control appetite could also fight anxiety
Did you know that our body produces its own marijuana-like...
Molecule from trees helps female mice only resist weight gain
A molecule found in some plants can combat weight gain...
Researchers discover protein's pivotal role in heart failure
Researchers at the University of California, San Diego School of...
From chick to bedside: Removing the Wnt barrier
Kick starting a process that might repair the damage done...
In vivo CRISPR-Cas9 screen sheds light on cancer metastasis and tumor evolution
For the first time, CRISPR-Cas9 gene-editing technology has been employed...
Estimated costs of endocrine-disrupting chemical exposure exceed $209 billion annually
A new economic analysis found exposure to endocrine-disrupting chemicals likely...
Phthalates potentially alter levels of a pregnancy hormone that influences sex development
Exposure to hormone-altering chemicals called phthalates - which are found...
The EdibleRx: Why Does Olive Oil Get All the Love?
(MedPage Today) -- Polyunsaturated oils like sesame and corn may...
Botox to improve smiles in children with facial paralysis
Injecting botulinum toxin A (known commercially as Botox) appears to...
Trends of 21-gene recurrence score assay use in older patients with breast cancer
A genetic test for patients with breast cancer that helps...
Effect of follow-up of MGUS on survival in patients with multiple myeloma
Patients with multiple myeloma (MM) appear to have better survival...
Researchers examine effect of experimental Ebola vaccine after high-risk exposure
A physician who received an experimental Ebola vaccine after experiencing...
Semi-veggie diet effectively lowers heart disease, stroke risk
A pro-vegetarian diet - one that has a higher proportion...

Privacy Statement - Copyright Information. - Contact Us

comments powered by Disqus

Integrated Publishing, Inc.
9438 US Hwy 19N #311 Port Richey, FL 34668

Phone For Parts Inquiries: (727) 755-3260
Google +