Over-staining (prolonged buffer action).
(d) Stain or diluent which is alkaline.
(e) Alkaline fumes.
(15) If the red blood cells are bright red, the stain is too acid. In this condition
they stain well but the white blood cells (except eosinophilic granules) stain very poorly
if at all. Thus, the stain is of no value for differential studies. ''Tendency toward acid
staining is caused by:
Incomplete drying before staining.
(b) Insufficient staining (insufficient buffer action).
Overdilution of the stain with buffer.
(d) Prolonged washing of the slide after staining.
(e) Stain or buffer which is acid.
(16) The staining reactions of blood are as follows:
Type of blood cell
Blue or green
Pink to orange Bright red
Brilliant red, distinct Deep gray or blue
Neutrophilic Granules Violet-pink
(17) The technician should strive for a staining reaction that is neither too
alkaline nor too acid. Such a stain gives good distinguishing features for all the cells of
the blood system.