Example
Mrs. K.M., 1970
1
2
4
8
16
32
64
128 Total Score
Strength of reaction
4
3
3
2
2
1
1
0
Score
12 10
10 8
8
5
5
0
58
Mrs. K.M., 1974
Strength of reaction
4
4
4
4
3
2
1
0
Score
12 12
12 12
10
8
5
0
71
The two serums have titers of 64, yet the difference in score indicates that there has
been an increase in antibody strength.
1-40. ABSORPTION
a. Background.
(1) Absorption is removing antibody from a serum by reacting it with the
specific antigen, and then physically separating the antigen- antibody complexes from
the serum. Intact RBCs are the usual source of antigen, but RBC stroma is
occasionally used. Situations in which absorption may be useful include:
(a) Removal of cold or warm autoantibody activity to permit evaluation
of coexisting alloantibody.
(b) Removal of anti-A and/or anti-B from serum containing an antibody,
suitable for reagent use.
(c)
Separation of mixed antibodies in serum, or eluate, as an aid to
identification.
(d) Documentation that cells contain an antigen, through demonstrating
their ability to remove antibody from serum.
MD0846
1-58
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