Reagent
Definition
Polyspecific
Contains anti-lgG and anti-C3d; may
contain other anticomplement and other
anti-immunoglobulin antibodies.
Polyspecific
Contains rabbit antihuman lgG and murine
monoclonal anti-C3b and -C3d.
Anti-lgG
Contains anti-lgG with no complement
activity.
Anti-lgG (heavy chain)
Contains only antibodies reactive against
human gamma chains.
Anti-C3d and Anti-C3b, Anti-C3d
Contains only antibodies reactive against
the designated complement component(s),
with no anti-immunoglobulin activity.
Anti-C3d (murine monoclonal)
Contains only antibodies reactive against
Anti-C3b, -C3d (monoclonal)
the designated complement component,
with no anti-immunoglobulin activity.
* As defined by the FDA: Code of Federal Regulations 21 CFR 660.
Table 1-2. Antihuman globulin reactions.
1-23. FACTORS AFFECTING THE ANTIGLOBULIN TEST
a. Sensitization Phase ("In Vitro" Only).
(1) Temperature. Incubation is normally at 37C, as most clinically
significant lgG antibodies react optimally at 37C. Complement sensitization also
occurs optimally at 37C.
(2) Medium. The suspending medium for the RBCs may be saline, albumin,
or low-ionic-strength saline (LlSS) serum. There is evidence that a shorter incubation
period can be used if albumin is present in the incubation mixture, and also that
uncommon antibodies are detected in the presence of albumin that fail to react when
RBCs are suspended in saline. Antibody association is considerably enhanced if RBCs
are suspended in a simple low-ionic-strength medium.
MD0846
1-28
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