Figure 3-5. Pour measured water sample into funnel.
(1) Sample greater than 20 ml: Use a sterile graduated cylinder to measure
the sample and pour it into the funnel.
(2) Sample of 2 to 20 ml: Pour about 30 ml of sterile buffered dilution water
into the funnel; then using a sterile 10- or 20-ml pipette, measure the sample and put it
into the funnel.
(3) Sample of 0.5 to 2 ml: Pour about 30 ml of sterile buffered dilution water
into the funnel; then using a sterile 1- or 2-ml pipette, 1-5 measure the sample and put it
into the funnel.
e. Connect the hand suction pump to the filter base and withdraw air by means
of a pumping action, thus aiding the sample to pass through the filter (see figure 3-6).
Figure 3-6. Apply suction to filter holder.
MD0160
3-19