Conjugates are e___e-labeled a___n or a____y which retain activity capable of
converting the substrate into a product that can be easily detected.
The conjugates (enzymes) most frequently used include h____sh peroxidase
(HRP) and alkaline p_____ase (AP). The most commonly used conjugate in the
EIA test procedures is horseradish p_____ase.
The second most commonly used conjugate is a____ine p____ase, which has a
greater resistance to environmental toxins and is derived from the mucosa of
the c__f i______s.
Substrates are c_____nic substances which are initially colorless but yield a
colored product after e____e degradation.
The substrates most frequently used in the EIA procedures are:
Ortho-phenylenediamine (OPD), the most s_____ory and commonly used
peroxidase substrate, which yields a highly c_______ic orange product after
enzyme degradation has occurred.
Para-nitrophenyl phosphate (p-NPP), the substrate used for the enzyme alkaline
phosphatase and which yields a highly chromogenic y____w-colored product after
enzyme degradation. Both of the above mentioned substrates are highly s___le
and extremely l___t sensitive.
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