(3) Inoculation, Incubation and Processing--use the same procedure as for
Boeck and Drbohlav's Medium.
c. Liver Infusion Solid Medium: (Cleveland and Collier, Cleveland and
Sanders, 1930). Entamoeba histolytica grows well in this medium while the other
intestinal protozoans do not. Therefore, it is useful as a selective medium and frequently
used in metabolic research laboratories.
d. Charcoal: (McQuay, 1956). This is a modified mycobacterial medium which is
excellent for the isolation and storage of amoebic organisms (e.g., Entamoeba hartmanii,
Dientamoeba fragilis, and to some extent Entamoeba histolytica). The charcoal medium
can be prepared as semi-solid slanted medium or in a liquid form.