(b) Procedure.
1 Prepare fresh smears. Do not use a fixed specimen.
2 Transfer the racks or slides from solution to solution,
remembering to drain them on paper towels between steps.
REAGENT
TIME
STEP 1: Stain Dilution .......................... 2 hours (24)
STEP 2: Ethanol, 95% .......................... 2-3 dips
STEP 3: Ethanol, Absolute ................... 3-5 dips
STEP 4: Carbol-Xylol............................ 3-5 minutes
STEP 5: Xylol ....................................... 3-5 minutes
3 Mount with a suitable medium.
2-20. STAIN REACTIONS
SALINE
IODINE
MIF
IRON
TRICHROME*
CBE
CYTOPLASM
colorless yellow to
pink
gray
blue-green
green-
tan
to gray
black
CYST WALL
refractile
dark brown
pink to red colorless colorless
green-
refractile
refractile
(halo)
black
NUCLEI
refractile
dark brown
dark red
black
dark red
green-
refractile
refractile
black
CHROMATOID refractile
dark brown
pink to red gray to
red
gray-
BODIES
refractile
refractile
black
black
GLYCOGEN
colorless mahogany
colorless
colorless colorless
color-
VACUOLES
brown
less
MD0841
2-55