b. Careful technique and complete concentration are necessary in testing, since
incorrect results can directly endanger the life of the recipient.
c. The AABB Standards and the FDA (BoB) require the testing of the donor's
cells with the recipient's serum, (major crossmatch), by a method that will demonstrate
agglutinating, coating, and hemolyzing antibodies, which, shall include the antiglobulin
method. Antihuman globulin reagent for the antiglobulin test shall meet FDA (BoB)
d. Testing donor serum with recipient RBCs (minor crossmatch) need not be
included, since donor serum must be tested initially for hemolyzing, agglutinating, and
coating antibodies with reagent RBCs, meeting FDA (BoB) standards.
e. The crossmatch will detect the following:
Most recipient antibodies, directed against antigens on the donor RBCs.
Major errors in ABO grouping, labeling, identification of donors, and
f. The crossmatch will not:
Guarantee normal donor cell survival.
Prevent recipient immunization.
Detect all ABO grouping errors.
(4) Detect Rh typing errors, (unless the recipient's serum contains Rh
antibody from previous immunization).
(5) Detect all unexpected RBC antibodies in recipient serum. Clerical errors
are more common than technical ones in a blood bank, or transfusion service.
Nontechnical mistakes, such as inadequate or incorrect identification of the recipient or
donor, are usually caused by not adhering to established protocols.
1-43. COLLECTION OF BLOOD FROM RECIPIENT
a. Blood request forms must include the recipient's full name, and identification
number, and because blood is a drug and for other medical-legal reasons, the name of
the responsible physician should appear on the requisition form. Additional information
such as sex, amount of blood, or component requested may be helpful, but this is not
required by the AABB Standards. Only completed forms may be accepted.
Transmittals from a computer program are acceptable. Telephone requests should be
confirmed in writing with a properly completed request form.