present in the eluate, hemolysis of the cells used in testing is not expected. Since
eluates may not be stable, it is preferable that they be tested on the day of preparation.
If storage is anticipated, eluates should be prepared in AB serum or 6 percent albumin
and frozen at -20C or lower.
c. Heat Elution.
(1) STEP 1. Add a volume of saline, (or group AB serum or 6 percent
(albumin), to each volume of packed, washed, RBCs.
(2) STEP 2. Mix well, and place in a 56C water bath, approximately 10
minutes, agitating frequently during incubation.
(3) STEP 3. Immediately transfer the tube to prewarmed centrifuge cups
containing 56C water.
STEP 5. Remove the hemoglobin-tinted supernatant eluate.
If care is taken and the cells agitated gently for five minutes, it is possible to
remove the antibody, and still have intact cells. The eluate is very slightly
Section IV. DETERMINATION OF COMPATIBILITY
1-42. DETERMINATION OF COMPATIBILITY
a. Compatibility testing consists of a series of procedures performed by the
blood bank, before transfusion, to ensure the proper selection of blood for the patient.
These procedures should include the following:
(1) A review, of the blood bank records, for results of previous testing to
check for the recipient's group, type, and for any unexpected RBC antibody that may
have been previously identified.
(2) ABO grouping, Rh typing, and red cell antibody detection on each
recipient sample, sent for compatibility testing.
(3) ABO grouping, Rh typing, and red cell antibody detection on the unit of
blood. (The antibody detection test need not be repeated if it has been performed by
the collecting agent.)