(b) Solution B.
Eosin Y, water-soluble ...................................... 1.0 gm
Distilled water .................................................... 500.0 ml
STEP 1: Dissolve the Eosin Y in 500 milliliters of distilled water and shake vigorously.
STEP 2: Allow the solution to age for 48 hours and filter before use.
(2)
Procedure.
STEP 1: Fix the thin smear and lake the thick preparation as done in the Giemsa stain
procedure.
STEP 2: Immerse the whole slide in solution A for 30 seconds.
STEP 3: Tap the slide on towels to remove the excess stain. Dip the slide five times in
a slightly acidified (pH 6.2-6.6) water solution.
STEP 4: Tap the slide on paper towels. Dip the slide twice into solution B.
STEP 5: Tap the slide on paper towels. Dip the slide five times in another jar with
slightly acidified water.
STEP 6: Immerse the slide in solution A for 30 seconds.
STEP 7: Tap the slide on paper towels. Dip the slide five times into acidified water.
STEP 8: Allow the slide to air dry and examine it under the oil immersion objective.
e. Knott's Technique for Examination of Microfilariae. The laking of blood
with two percent formalin solution followed by centrifugation will often reveal
microfilariae when present only in small numbers. Staining of the blood smears clarifies
the morphological detail of the microfilariae.
(1)
Preparation of solutions.
(a) Ehrlich's Hematoxylin Stain.
1 Solution A.
Hematoxylin crystals .................................... 1 gm
Absolute ethanol .......................................... 10 ml
Glacial acetic acid ........................................ 10 ml
MD0841
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