3 In this example, the serum could be absorbed with rh" (E)-
positive, Le (a-b-) RBCs, and the anti-rh"(E) eluted from them. The absorbed serum
should still contain anti-Lea. Absorption and elution of Lewis antibodies is not generally
satisfactory. The activity of the anti- Lea may be inhibited by using saliva or serum from
a Lewis positive, preferably Le (a+) person. Although Le(a+) people are ABH
nonsecretors, they have Lewis substance in their body fluids.
e. The serologic behavior of antibodies of a single specificity may vary from one
serum to another. Table 1-6 lists some examples of these. Serum containing more
than one antibody might give complex results; absorption, elution, and testing against
specially selected cells may be necessary for final identification.
Temperature
Blood Samples ( percent)
Technique
(C)
0-10
10-50
50-75
75-90
90-100
Lua
Lea
Leb
Saline and/or
4-22
MN
I
albumin
i
P1
rh (Cw)
w
22-37
rh"(E)
rh'(C)
RhO(D)
hr"(e)
Lua
Lea
Leb
hr'(c)
k U
S
s
Vel
rhw(Cw)
Saline and/or
37
rh"(E)
rh'(C)
RhO(D)
hr"(e)
Lea
Leb
albumin plus
K
hr'(c)
k U
Kpa
Fya
Fyb
KpbGea
antiglobulin
S
Lua
Jsa
Jkb
LubYta
s
Dia
Xga
Jka
Vel Jsb
Ytb
Doa
Dib
Au
rhw(Cw)
Enzyme
37
rh"(E)
rh'(C)
RhO(D)
hr"(e)
Lea
Leb
hr'(c)
rhw(Cw)
Enzyme plus
37
rh"(E)
rh'(C)
RhO(D)
hr"(e)
Lea
Leb
hr'(c)
antiglobulin
JKb
JKa
Doa
Lea
Leb
JKa
Hemolysis
37
Vel
JKb
Saline
Lea
Leb
JKa
Hemolysis
37
JKb
P1
Enzyme
Lea
Leb
PP1Pk
All techniques &
temperatures
MD0846
1-45