Patient's Group: O rh (dce)
Antigenic Determinants
Results
RhO rh' rh" hr' hr"
Saline
En-
K k Fya Fyb
Panel cell
D
C
E
c
e
37C HP
AGT Enzyme
1.
+
0
0
+
+
0
+
0
0
0
+
3+
3+
2.
0
0
0
+
+
+
+
0
+
0
0
0
0
3.
0
+
0
+
+
0
+
+
0
0
0
0
0
4.
+
+
0
0
+
0
+
+
+
0
0
3+
3+
5.
+
0
+
+
0
0
+
+
0
+W
2+
4+
4+
6.
0
0
+
+
+
0
+
0
+
0
0
0
0
Autologous 0
0
0
+
+
0+0
+
0
0
0
0
cells
0 = no agglutination; HP = high protein; AGT = antiglobulin test
Table 1-4. Example of a single antibody identified with a simplified cell panel.
(a) STEP 1. Cross out all antigenic determinants present in the first
RBC sample that did not react with the serum. In this example, number two.
D C E c e K K Fya Fyb
(b) STEP 2. Repeat with the next negative sample. In this example,
number three thus eliminating rh' (C) and Fya.
D C E Fya
(c) STEP 3. Repeat with the next negative sample, in this example,
number six, thus eliminating rh" (E).
DE
(d) STEP 4. Consider only the antigenic determinant that has not been
crossed out, D.
1 Do all the cells reacting possess this determinant? Yes.
2 Do all cells possessing it react? Yes.
3 Does the patient lack it? Yes.
(e) STEP 5. Antibody identification: Anti-Rho (D).
MD0846
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