3 Stain.
Chromothrope 2R ........................................ 0.6 gm
Light Green SF ............................................ 0.15 gm
Fast Green................................................... 0.15 gm
Phosphotungstic Acid .................................. 0.7 gm
Glacial Acetic Acid ....................................... 1.0 ml
Distilled Water.............................................. 100.0 ml
Mix the stains with the acetic acid and allow to stand at room
temperature for 30 minutes. Add the water and mix vigorously.
4 Acidified 90% ethanol.
Ethanol, 90% ............................................... 99.55ml
Glacial acetic acid ........................................ 0.45 ml
Add the acid to the alcohol. Do not use 95 percent ethanol.
(b) Procedure.
NOTE:
Prepare smears and staining tray as done for the iron hematoxylin procedure.
NOTE:
Transfer the slides or racks from dish to dish. Remember that the slides or
racks must be drained on paper towels between steps.
MD0841
2-52