NOTE:
To prevent transfer of a previous solution, it is necessary to drain the rack or
slides on paper towels prior to proceeding to the next reagent.
REAGENT
SOLUTION
TIME (MIN)
STEP 1: Working Iodine Alcohol.............................A ......................................... 5
STEP 2: Ethanol, 50% ........................................................................................ 3
STEP 3: Tap Water ............................................................................................ 3
STEP 4: Mordant ....................................................B ......................................... 3-5
STEP 5: Tap Water ............................................................................................ 1
STEP 6: Working Hematoxylin Stain ......................C ......................................... 1
STEP 7: Tap Water ............................................................................................ 1
STEP 8: Phosphotungstic Acid...............................D ......................................... 2-4
STEP 9: Running Tap Water .............................................................................. 5
STEP 10: Ethanol, 705 with Lithium Carbonate .......E ......................................... 3
STEP 11: Ethanol, 95% ........................................................................................ 5
STEP 12: Xylol ..................................................................................................... 3
(c)
Mount using a suitable mounting medium.
(2) Trichrome. The Gomori-Wheatley procedure is a very rapid and useful
method for the identification of amoebic organisms. This is a multi- color stain that
requires no differentiation step. Fresh PVA-fixed smears require increased timing and
the fixation step is omitted.
(a) Reagents.
1 Schaudinn's fixative--solution A. Same as above.
2 Working iodine alcohol--solution B. Same as above.
MD0841
2-51