MATERIAL EMPLOYED IN HEMATOLOGY
Section I. LABORATORY REAGENTS
Various stains and solutions are utilized in routine hematological examinations.
These stains and solutions must be prepared with the utmost care and precisely
according to formulations. Detailed directions for the preparation of all reagents that are
required for performing procedures outlined throughout this subcourse are contained in
the respective procedure. Careful attention should be given to precise measurements
order in which reagents are added, control of temperature where indicated, filtration,
and aging. Particular attention must be given to storage of reagents particularly with
reference to requirements for refrigeration, incubation, and protection from intense light.
LABELING REAGENT CONTAINERS
Proper labeling of reagents is an extremely important detail. Labels should be
complete, securely attached, and neatly and legibly written or preferably typewritten.
Items recorded on the label should include all constituents and quantities utilized, date
of preparation, initials of the individual who prepared the reagent, and expiration date if
the solution deteriorates with age. Labels should be protected against damage from
water or other fluids by covering with a protective coating of cellophane tape over the
surface of the label.
There are various precautions that must be taken in handling reagents in the
hematology laboratory. Among the most important are the following:
a. Once a portion of a reagent has been removed from the original container, it
should never be poured back because it can contaminate the remaining reagent.
b. Reagents are preferably stored in alphabetical order on shelving protected
from dust, moisture, and direct sunlight.
c. Never use a reagent that cannot be clearly identified from the label on the
container. Discard all reagents that cannot be accurately identified.
d. Always read the label before dispensing a reagent.
e. When working with newly prepared reagents, especially stains, ascertain
whether desired results are being obtained. Unsatisfactory solutions should be
discarded and replaced.