f. All pipets should be inspected each time before use so as to prevent the use
of equipment that is dirty or that has chipped ends.
a. The Unopette System (Becton- Dickinson and Co.) consists of a disposable
uniform-bore glass capillary pipet with an attached plastic tab for handling. The pipet
(see figure 2-2) is attached to a plastic reservoir in which predetermined amounts of
diluting fluids, depending on their purpose, can be placed. The blood aspirated into the
diluting fluid can be mixed and dispensed with great ease and speed.
Figure 2-2. Unopette System.
b. The Unopette System can be used for a variety of hematological procedures.
In general, the Unopette System is used as follows:
(1) Puncture diaphragm. Using the protective shield on the capillary pipette,
puncture the diaphragm of the reservoir as follows:
(a) Place reservoir on a flat surface. Grasping reservoir in one hand,
take pipette assembly in other hand and push tip of pipette shield firmly through
diaphragm in neck of reservoir, then removed.
Figure 2-3. Unopetted filling procedure (continued)