(d) Cover opening of overflow chamber of pipette with index finger and
seat pipette securely in reservoir neck.
Figure 2-3. Unopetted filling procedure (continued)
(e) Release pressure on reservoir. Then remove finger from pipette
opening. Negative pressure will draw blood into diluent.
(f) Squeeze reservoir gently two or three times to raise capillary bore,
forcing diluent up into, but not out of, overflow chamber, releasing pressure each time to
return mixture of reservoir.
Figure 2-3. Unopetted filling procedure (continued)
(g) Place index finger over upper opening and gently invert several
times to thoroughly mix blood with diluent.
Figure 2-3. Unopetted filling procedure (concluded).
MD0853
2-7