(5) Clamp the dispenser tube with a compression type clamp or forceps
approximately 4 inches from the free tip of the dispenser tube.
(6) Sanitize the distal 3 inches of the free end of the dispenser tube
thoroughly using a sterile swab saturated with 70 percent isopropyl alcohol.
(7) Sanitize the knife, razor blade, or other cutting device to be used for
severing the dispenser tube by alcohol-flaming the instrument or by applying an equally
acceptable germicidal treatment.
(8) Sever the dispenser tube in the alcohol cleansed area approximately 1
inch from the free end, using a technique that precludes contamination of the dispenser
tube by the fingers. Use of a second pair of hemostatic forceps and cutting between the
two forceps will eliminate unnecessary movement of the tubing. Use a technique that
(9) Extract a minimum of 1 pint (16 fl oz) of product through the dispenser
tube prior to collecting the sample to be forwarded for laboratory testing. Such a
procedure is necessary to ensure that the sample is obtained from the agitated contents
of the container. Also, this initially extracted quantity of the product may be used for
delivery temperature determinations and also sensory evaluation when sampled at
(10) Relock the clamp on the dispenser tube.
b. Collecting the Sample.
(1) Aseptically remove the lid from a sterile sample bottle, which has a
minimum capacity of 8 fluid ounces. If the inner surfaces of either the lid or the bottle
becomes contaminated, a second sample bottle must be used and the first bottle
(2) Release the clamp so that milk flows freely through the dispenser tube.
(3) Immediately fill the sample bottle to near capacity by releasing the clamp
or forceps and allowing milk to flow through the dispenser tube into the bottle.
(4) Relock the clamp on the dispenser tube.
(5) Carefully replace the sample bottle lid in a manner which precludes
possible contamination. After the lid has been securely replaced, further seal the
sample bottle by applying masking tape around the juncture of the bottle and lid to
eliminate the possibility of the lid being loosened during transit as a result of vibration.
(6) Reseal the dispenser tube by folding the tube approximately 2 inches
from the cut end, and then apply a piece of adhesive tape or other suitable material