EXERCISES, LESSON 6
INSTRUCTIONS: Answer the following items by completing the statement or by writing
the answer in the space provided.
After you have completed all of these items, turn to "Solutions to Exercises" at the
end of the lesson and check your answers with the solutions.
Immunofluorescence is a method of detecting an a___n or a___y in tissue by the
pattern of f_______e resulting when the tissue is exposed to the specific antibody
or antigen-labeled with a f____me such as fluorescein. Immunofluorescence can
be q____tive or q___tive. In a qualitative procedure, a f____t microscope is
necessary to visualize the presence of a labeled antigen or antibody in the
specimen. Fluorescence is the e____n of light of one color from a substance
being exposed to l____t of a different color or wavelength. One of the most
common fluorochromes is f_______n isothiocyanate.
The two most common methods used in the performance of immunofluorescent
microscopy are the d___t and i_____t techniques.
In the direct method, the antibody is labeled with a fluorescent compound and is
used to detect the presence of a___n in tissue fixed to a slide. The direct
technique utilizes b___sy material obtained from a patient. The fluorescent-
labeled a___s are added to the antigen in an optimal dilution and allowed to react.
The preparation is washed to remove any unr____d labeled antibodies. The
tissue sections are blotted and the preparation mounted with buffered g____l for
examination with the fluorescent microscope.
The indirect method is used for the detection of serum a_____s utilizing an
a_____n-containing substrate and a f_____n-labeled antibody specific for human
imm_____s. The specific antigen-antibody (unlabeled) reaction may be visualized
by the addition of labeled a_____n globulin directed against the antibody in the
primary reaction. The antigen substrate plus patient's serum antibody plus
labeled anti____n i_____globulin complex results in f_____e and detection of the
specific patient's a____y in question.