g. Calculations.
(1)
Number of sperm
counted X dilution (20)
= sperm per cu mm
volume (0.4)
(2)
Sperm per cu mm X 1000 = sperm per ml.
h. Sources of Error.
(1)
Delay in analysis results in a lower percentage of motile forms and a
lower count.
I
(2)
Temperature extremes cause spermatozoa to die.
(3)
See paragraph 5-2e for sources of error when counting.
i. Discussion.
(1) Semen analyses are usually performed as part of infertility studies or
following a vasectomy.
(2) Semen analysis can be performed for medico-legal cases involving rape
or to support or disprove a denial of paternity on the grounds of sterility.
(3) Semen is derived from the following: testes, seminal vesicles, prostate,
epididymides, vasa deferentia, bulourethral glands, and urethral glands.
j. Normal Values.
Volume: 1.5-5.0 ml.
(1)
(2)
pH: 7.2-7.6.
(3)
Motility: 60-90 percent.
(4)
Spermatozoa Count: 60-150 million per ml.
MD0853
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