Figure 5-2. Miller disc.
g. Normal Values.
(1) Birth. Two and one-half to 6.0 percent, but falls to adult range by the
end of second week of life.
Adults (both sexes). Five-tenths to 1.5 percent.
Section II. MANUAL COUNTS, OTHER BODY FLUIDS
CEREBROSPINAL FLUID CELL COUNTS
a. Principle. Cerebrospinal fluid is delivered to a counting chamber and
examined microscopically for blood cells. Normally, spinal fluid is clear. If it is cloudy,
dilute before charging the counting chamber.
b. Reagent. Cerebrospinal Fluid Diluting Fluid: Add 10 ml of glacial acetic acid
and 0.2 grams of crystal violet to a 100-ml volumetric flask. Dilute to the mark with
Set up cell counts on spinal fluids within 30 minutes after withdrawal of the
Clear spinal fluid is set up as follows:
(a) With a capillary pipet introduce a drop of well-mixed spinal fluid into
one counting chamber of a hemacytometer.