(3) Repeat this procedure in three hours and six hours, using a new drop
from the original specimen each time.
f. Spermatozoa Count.
Draw the liquefied semen to the "0.5" mark on white blood cell diluting
Draw fixative solution to the "11" mark on the WBC pipet .
Let the mixture stand until the mucus dissolves.
Shake the pipet thoroughly and charge a hemacytometer.
Count the spermatozoa in the same manner as you would count white
(6) After counting the sperm, examine the morphology and report the
percent of abnormal forms. Morphologically normal sperm are quite uniform in
appearance. Any sperm with rounded, enlarged, small, or bilobed heads are abnormal.
Abnormal tails are enlarged, small, irregular in length, absent, or multiple. See
Figure 5-3 for morphology of spermatozoa.
Figure 5-3. Morphology of spermatozoa.